Human (human) myocardial antibody (AMA)
This reagent is for research use only: Serum 1. Reagent composition
1. Microtest Plates 96 wells 2. Enzyme Conjugate 12.0ml
3. Standard 5 x 1.0ml4, Color A, Substrate A 6.0ml
5. Reagent B, Substrate B 6.0ml 6, Stop Solution 6.0ml
7. Concentrated washing solution (1:20) Rinsing Buffer 60.0ml 8, 5 times sample dilution solution 15.0m
2. Matters needing attention
1. This reagent is an in vitro test reagent. It should be used within the validity period. The reagent should be regarded as an infectious agent.
2. Before use, remove all reagents from the box and leave at room temperature for at least 30 minutes.
3. Store at 2-8 ° C, do not freeze, please see the label in the box for the expiration date.
4. After the concentrated washing liquid crystallizes, please incubate at 37 ℃ for 15 minutes
3. Operation steps
1. Add 100ul of diluted sample and standard to each well.
2. Incubate the plate at 37 ° C for 30 minutes.
3. Shake off the liquid in the plate, wash with the application of washing liquid, stay for 30 seconds each time, pat dry on paper. Repeat the operation 5 times.
4. Add 100ul of enzyme-labeled coupling solution to each well.
5. Incubate the plate at 37 ° C for 30 minutes.
6. Shake off the liquid in the board, wash with the application of washing liquid, stay for 30 seconds each time, pat dry on paper. Repeat the operation 5 times.
7. Add 50 ul each of Substrate A and Substrate B to each well and place in a 37 ° C incubator for 15 minutes.
8. Add 50ul of stop solution to each well and read the OD value at 450nm.
4. Result judgment instrument value: Read the OD value of each well on a microplate reader with a wavelength of 450nm.
Detection value range: 0 – 240pg / ml
Sensitivity: 1.0pg / ml
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