The ELISA experimental procedure described here follows the double-antibody sandwich method, a widely used and reliable technique for quantifying specific analytes in biological samples. This method involves immobilizing a capture antibody on a solid surface, followed by the addition of the sample, allowing the target antigen to bind. A detection antibody, conjugated with an enzyme, is then added to form a complex, which is visualized through a colorimetric reaction.
Guangrui Bio, a leading domestic supplier of high-quality ELISA kits, offers a wide range of products designed for accurate and efficient detection of various biomarkers. These include kits for measuring major histocompatibility complex class I (MHCI/HLAI), corticosterone (CORT) from the adrenal gland, neutrophil chemoattractant protein 2 (NAP-2/CXCL7), monocyte chemotactic protein 4 (MCP-4/CCL13), anti-parainfluenza virus IgG antibodies, matrix metalloproteinase 8 (MMP-8), thyrotropin (TSH), PL7 antibody, Orexin A, connective tissue growth factor (CTGF), brain natriuretic peptide (BNP), von Willebrand factor (VWF), urokinase-type plasminogen activator (uPA/PLAU), anti-glycoprotein antibodies, mannose-binding protein (MBP/MBL), intestinal fatty acid binding protein (iFABP), anti-Rubella virus IgM antibodies, free thyroid hormone (FT4), HSVII-Ab, insulin autoantibodies (IAA), anti-malabsorption protein/gliadin antibodies (AGA), complement 1 inhibitor (C1INH), and vascular endothelial growth factor B (VEGF-B).
Each kit comes with detailed instructions, ensuring proper handling, preparation, and interpretation of results. Whether you are working with mouse, rat, rabbit, or human samples, these ELISA kits provide consistent and reproducible data. They are ideal for research in immunology, endocrinology, neurobiology, and inflammatory diseases. By using these high-quality reagents, researchers can obtain precise measurements of key biomolecules, supporting both basic and translational studies.
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