Mix 3.0 ml of the sample with 10 ml of reagent 1. Then, add 0.5 g of reagent 2 and shake the mixture thoroughly to ensure complete dissolution. Next, add 10 ml of reagent 3 and mix well. After mixing, filter the solution using a fluted filter to remove any carbon particles. Once filtered, measure the clear, particle-free solution against distilled water at a wavelength of 445 nm using a spectrophotometer. This procedure ensures accurate optical density readings by eliminating potential interferences from suspended materials. Make sure all steps are performed carefully to maintain consistency and reliability in the results.
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