Shanghai Haozhuang Instrument specializes in the production of a wide range of anaerobic incubators, also known as anaerobic workstations or anaerobic glove boxes. These devices are essential tools for bacterial culture and manipulation under strictly anaerobic conditions. They maintain a stable anaerobic environment with precise temperature control and provide a safe, systematic workspace for scientific research.
### 1. Equipment Overview
**1.1 Equipment Name**
An anaerobic incubator is a specialized device used to cultivate bacteria in an oxygen-free environment. It ensures a consistent anaerobic state, making it ideal for working with sensitive anaerobic organisms that cannot survive in the presence of oxygen.
**1.2 Scope of Application**
This equipment is designed for microbiological research and applications where anaerobic conditions are critical. It enables the cultivation of even the most challenging anaerobic species while protecting them from exposure to atmospheric oxygen, which could be lethal.
**1.3 Structure and Function**
The anaerobic incubator typically includes a constant-temperature culture chamber, an anaerobic operation room, a sampling chamber, gas and electrical control systems, a frame, bottle holders, and a wax sterilizer. Electrorotek, a leading expert in anaerobic technology, has improved the design to make it more user-friendly and efficient.
The modern design consists of four key components: the anaerobic chamber, the transfer chamber, the bare-hand sleeve access port, and the gas and control system.
- **Anaerobic Chamber**: Equipped with mechanical forced convection and positive pressure, it maintains uniform temperature, humidity, and oxygen levels, allowing for rapid recovery and continuous operation.
- **Transfer Chamber**: A compact, cylindrical design simplifies sample handling and transfers.
- **Bare-Hand Sleeve Access Port**: Eliminates the need for vacuum or nitrogen replacement during hand insertion, ensuring quick and automatic restoration of anaerobic conditions.
- **Control System**: Features real-time monitoring, alarms, and automated functions to ensure smooth and safe operation.
**1.4 Main Features**
- Compact design suitable for small laboratories.
- Fully automated operations, including temperature, humidity, and anaerobic state control.
- Allows bare-hand operation, reducing discomfort from heavy gloves.
- Ergonomic design reduces strain during long-term use.
- Complete standard components reduce post-purchase concerns.
**1.5 Installation Notes**
- Place the unit in a stable location away from direct sunlight and heat sources.
- Secure gas cylinders and install pressure regulators properly.
- Check all gas connections for leaks and seal as needed.
### 2. Operating Instructions
**2.1 Creating an Anaerobic Environment**
1. Prepare necessary items and place two poison bags inside the operation chamber.
2. Adjust gas cylinder and nitrogen pressure to 0.1 MPa.
3. Power on the device and activate the main power switch.
4. Add palladium particles and desiccant, along with a methylene blue indicator strip.
5. Close doors and perform a vacuum check.
6. Conduct three nitrogen flushes to remove oxygen.
7. Replace with a mixed gas (Hâ‚‚: 10%, COâ‚‚: 15%) for final purification.
8. Activate the deoxidizer and monitor the indicator strip for color change.
9. Use UV lamps for sterilization as required.
**2.2 Inoculation and Culture**
1. Ensure the sampling chamber door is closed.
2. Insert the culture into the sampling room and close the outer door.
3. Perform three nitrogen flushes to purge the sampling chamber.
4. For long-term use, monitor the anaerobic status daily and replace deoxidizers regularly.
5. Use the inoculation rod sterilizer for proper sterilization.
6. Control the incubator temperature as needed.
**2.3 Safety and Maintenance Tips**
- Install the meter in a stable, quiet area.
- Familiarize yourself with all instruments before use.
- Only start cultures once the anaerobic environment is confirmed.
- If a malfunction occurs, the chamber can maintain anaerobic conditions for up to 12 hours.
- Regularly inspect the gas lines and tighten connections.
- Turn off the device when not in use.
**2.4 Common Faults and Solutions**
| Problem | Cause | Solution |
|--------|-------|----------|
| No power | Loose plug, blown fuse, faulty switch | Plug in, replace fuse, replace switch |
| Incubator won’t open | Low set temperature, faulty probe, leakage | Adjust temperature, replace probe, check seals |
| Gas path blocked | Broken valve, leaking tube, empty cylinder | Replace valve, fix tube, refill gas |
| Light not working | Failed starter, burned rectifier | Replace starter or rectifier |
Shanghai Haozhuang Instrument Co., Ltd. offers advanced production technology and professional support. Contact us for more information.
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