Brief introduction of ultrasonic cell disruptor

1. Working principle

The working principle of the ultrasonic cell breaker is based on the cavitation effect of ultrasonic waves in the liquid. The transducer will generate electrical energy through the horn to produce high-strength shearing force in the liquid at the top of the tool head, forming high-frequency alternating water pressure, which The cavity expands and explodes to break up the cells. In addition, due to the violent disturbance caused by the ultrasonic waves propagating in the liquid, the particles are greatly accelerated, colliding with each other or colliding with the walls to break or reorganize.

2. Application

It is widely used in the crushing, emulsification, separation and homogenization of animal and plant tissues, viruses, bacteria and other cells. Widely used in life sciences, pharmaceuticals, new materials and other fields.

Three, matters needing attention

1. Be sure to insert the horn of the ultrasonic cell disruptor into the sample before turning it on to prevent empty load.

2. The probe of the ultrasonic cell disruptor should be centered in the water and not attached to the wall. Ultrasonic waves are longitudinal waves. Inserting too deep will not easily form convection and affect the crushing efficiency.
3. Parameter setting of ultrasonic cell disruptor

1) Time: The time setting should be based on the principle of short ultrasound time and many ultrasound times, which can extend the life of the ultrasound machine and probe.
2) Ultrasonic power: it should not be too large to avoid splashing or foaming of the sample.

3) Container selection: The amount of sample determines the size of the container, which is conducive to convection of the sample in ultrasound and improves the crushing efficiency.
4. Daily maintenance: After use, scrub the probe with alcohol or perform ultrasound with clean water!

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