Experimental principle of human anti-steroid-producing cell antibody (SCA) ELISA kit

Experimental principle of human anti-steroid-producing cell antibody (SCA) ELISA kit

English name: Human steroid producing cell autoantibody, SCA ELISA
1. The level of human anti-steroid-producing cell antibody (SCA) in the specimen was determined by the double antibody sandwich method.
2. First coat the microplate with purified human anti-steroid producing cell antibody (SCA) antibody to make a solid phase antibody.
3. Then add anti-steroid-producing cell antibody (SCA) to the monoclonal antibody-coated microwells in sequence, and then combine with HRP-labeled anti-steroid-producing cell antibody (SCA) antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, add substrate TMB to develop color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the anti-steroid producing cell antibody (SCA) in the sample.
5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of human thyroid non-peptide hormone antibody (THAA) in the sample is calculated by a standard curve.
Objective: To determine the level of non-peptide thyroid antibody (THAA) in human serum, plasma and related fluid samples.

6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
Precautions:
1. There must be a complete plan before collecting specimens, and it must be clear whether the components to be tested are sufficiently stable.

2. It is recommended that fresh specimens be tested as soon as possible, and timely testing after collection. Samples tested in about 1-3 days can be stored at 4 ℃ for later use. If you need to collect specimens periodically for special reasons, please pack the specimens in a timely manner and store them at -20 ℃ or -70 ℃ to avoid repeated freezing and thawing.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children's own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months Product range:
Human, mouse, rat, pig, rabbit, other animal cytokines; apoptosis, active peptides, autoantibodies, thrombosis and hemostasis,
Bone metabolism, liver fibrosis, tumor, hormone endocrine, autoantibody scientific research ELISA test kit Shanghai Yanjin Biotechnology Co., Ltd. specializes in selling various brand price grade ELISA kits. Serving universities and immunology research units. Quality assurance and perfect after-sales service. And can be free to detect, better service for you.

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