THE COMPOSITION OF MEDIA
Medium and ingredients
1. Acetobacter Medium
Glucose (glucose) 100g Yeasst extract (yeast extract) 10g
CaCO3 20g Agar (agar) 15g
Distilled water 1000ml Adjust pH to 6.8
Scope of application: odorous variant of Acetobacter aceti
2. Nutrient Agar (Nutrition Gravy Agar)
Pepton 5g Beef extract 30g
NaCl 5g Agar (agar) 15g
Distilled water Adjust pH to 7.0-7.2
[Note]:When cultivation of Bacillus, 5mg of to MnSO4.H2O may be added .
It is favorable to promote spore formation .
Scope of application: gas-producing bacteria, Alcaligenes faecalis, Bacillus cereus, Bacillus cereus spp., Bacillus licheniformis, Bacillus megaterium, Bacillus polymyxa, Bacillus licheniformis, Bacillus pumilus, thermophilic Bacillus stearothermophilus, Bacillus subtilis, Bacillus subtilis deep black variety, Bacillus thuringiensis, Bacillus thuringiensis subsp. parasitic (C. sinensis), Bacillus thuringiensis Galsted variety, Bacillus thuringiensis subsp. subsp. Brevibacterium, Brevibacterium flavum, Corynebacterium glutamicum, Corynebacterium sinensis, Escherichia coli (E. coli), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Pseudomonas syringae, Pseudomonas fluorescens , Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas, Sarcina sphaeroides, Arborvitae, U. serrata, Staphylococcus aureus, Saccharomyces cerevisiae
Maize flour 5g Peptone 0.1g
Glucose (glucose) 1g Tap water (tap water) 1000ml
[Note]:Boil the mixture in autoclave at 121°C for 1 hr. distribute the medium
Into 18x18 mm tubes , each contains 10 ml of the liquid , then autoclave at
121 ° C for 1 hr . again (15 liters for 1 hour, divided into 18 x 18 mm tubes, each tube is 6 cm deep. 15 lbs are sterilized for another 15 hours.)
5. Lactic-bacteria Medium I (Lactic Acid Bacteria Medium I)
Yeast extract 7.5g Peptone 7.5g
Glucose (glucose) 10g KH2PO4 2g
Tomato juice 100ml Tween 80 0.5ml
Distilled water 900ml pH 7.0
Scope of application: Lactobacillus plantarum (L. lactis), thermophilic lactic acid streptococci
6. Lactic-bacteria Midium II (Lactic Acid Bacteria Medium II)
Lacto-casein peptone 10g Beef extract 10g
Yeast extract 5g Glucose (glucose) 5g
Tween 80 1g K2HPO4 2g
Na-acetate 5g Diamine citrate 2g
MgSO4.7H2O 0.2g MnSO4.H2O 0.05g
Distilled water 1000m pH 6.5-6.8
Scope of application: Lactobacillus plantarum (L. lactis)
7. Peotone Glucose Yeast extract Medium PGY (peptone, yeast extract, glucose medium)
Peptone 10g Yeast extract 5g
Glucose (glucose) 1g Distilled water (distilled water) 1L
8. Glycerol Agar (glycerol agar)
Peptone 5g Beef extract 3g
Glycerol (Glycerin) 20g Top water (tap) 1000ml
Agar (agar) 15g pH 7.0-7.2
9. Rhizobium medium AS 9
Yeast eztract (yeast extract) 1g Soil eztract (soil extract) 200ml
Mannitol 10g Agar (agar) 15g
Distilled water 800ml pH 7.2
[Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap
Autoclave at 121°C for 1 hr. Decant through cotton-cloth, filler though
Paper, make up volume to 200ml. Resterilize for 20 minutes at 121°C , then
Mixed with othringredients and distributed.
(Method of preparing soil extract: take 50 grams of soil, add 200 ml of water, cook for 15 hours at 15 lbs, filter with filter paper and add water to make 200 ml.)
Scope of application: soybean rhizobium (slow-type), cowpea slow-growing rhizobium, peanut rhizobium, Astragalus rhizobium,
Soybean rhizobium (fast-growing), soybean rhizobium, pea rhizobium, rhizobium, and rhizobium
10. Mannitol Agar (Mannitol Agar)
Yeast extract 5g Peptone 3g
Mannitol 25g Agar (agar) 15g
Distilled water 1000ml 11. Glucose Asparagine (glucose, asparagin agar)
Glucose (glucose) 10g Asparagine (asparagine) 0.5g
K2HPO4 0.5g Water (water) 1000ml
pH 7.2-7.4
Scope of application: Pseudomonas aeruginosa blush, purple micromonospora (monosporium)
12. Gause's Synthetic Agar (Gao's Synthetic No. 1 Agar)
KNO3 1g Soluble starch 20g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4 0.01g
Agar (agar) 20g water (water) 1000ml
pH 7.2-7.4
Scope of application: Pseudomonas aeruginosa blush variant, Pseudomonas aeruginosa (S. erythropolis), Streptomyces fulvum, Streptomyces cerevisiae, Streptomyces faecalis, Streptomyces faecalis, Streptomyces faecalis, cockroach Streptomyces cinerea, Streptomyces coelicolor, Streptomyces mosquito, Streptomyces erythromycin, Streptomyces erythromycin, Streptomyces coelicolor, Streptomyces spp., Streptomyces griseus, Streptomyces griseus, Streptomyces hygroscopicus, Streptomyces glabrata, yellow long Streptomyces spp., Streptomyces fulvum, Streptomyces fulvum, Streptomyces faecalis, Streptomyces roseosporus, Streptomyces faecalis, Streptomyces faecalis, Streptomyces venezuela, Streptomyces faecalis, Streptomyces lividans, Streptomyces lividans
13. Wort Agar (Wort Agar)
Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the
Diluted word..Melt the agar by heating, then distribute the medium into tubes.
Autoclave at 110 for 30 minutes.
(Diluted raw material of fermented beer (without hops), diluted to 12 Berlin, added 15 grams of agar, dissolved and dispensed. 15 lbs of sterilization for 30 minutes.)
Scope of application: Candida krusei, Candida utilis, Candida lipolytica, Candida macedonia, Candida tropicalis, Candida utilis, Candida rugosa, Candida tropicalis, Candida utilis, A. pseudoacacia, Geotrichum candidum, Geotrichum candidum, Geotrichum, Abnormal Hansenula, Hansenula variant, Arabidopsis hansenii, Hansenula serrata, sac Yeast, Pichia boringii, Pichia pastoris, Rhodotorula, Rhodotorula, Rhodotorula, Rhodotorula, Carlsberg, Saccharomyces cerevisiae, Saccharomyces cerevisiae, Fermentative yeast, Roche , Rosin yeast, R. solani, R. stipitis, Wilford yeast, yeast, Lutheran yeast, Schizosaccharomyces pombe, Thrushing yeast, Bacillus cerevisiae, Trichosporon cerevisiae, Aspergillus fumigatus, Aspergillus fumigatus, Aspergillus oryzae, Monascus, Aspergillus oryzae, Monascus red, Monascus
14. Potato Dextrose Agar PDA (potato, dextrose agar)
Potato extract 1000ml Dextrose(glucose) (glucose) 20g
Agar (agar) 20g
[Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately
To prevent Oxidtion of juice and boil until soft. (approximately 30min.).
Filter through cotton-cloth. Autoclave at 115°C for 20
Minutes. (Note: Take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, make up the filtrate to 1000 ml, add 20 g of glucose, 15 g of agar, dissolve and disperse. Sterilize for 15 minutes for 15 minutes.)
Scope of application: Pseudomonas aeruginosa (F. faecalis), Streptomyces faecalis, Streptomyces faecalis, Streptomyces coagulans, Streptomyces spp., Streptomyces griseus, Streptomyces griseus, Pythium oxysporum, elegant radiation Mucor, Aspergillus,
Aspergillus oryzae, Aureobasidium pullulans, Beauveria bassiana, Botrytis cinerea, Cephalosporium acremonium, Brazilian hair shell, long thorn shell, olive coat shell, recurved shell, amber shell, round leaven shell, bundle Spinosa spore, Curvularia lunata, singular wing spore, ground
Phytophthora, Fusarium oxysporum, Fusarium oxysporum, Phytophthora ssp. (Lubao No.1), Phytophthora sinensis, Helminthosporium fulvum, Mortierella aurantium, Mortierella aeruginosa, polyporus Mormanella sinensis, Mortierella aureus, Mucor sphaeroides, Mucor sinensis, Mucor sphaeroides, Mucor erectile, Mucor spp., Mucor sphaeroides, Mucor, Mucor, Mucor sclerophyllum, Mucor sclerophyllum, Muscle chinensis, Rhodopseudomonas sphaeroides, Rhodococcus sphaeroides, Paecilomyces sphaeroides, Paecilomyces militaris, Paecilomyces militaris, Fusarium oxysporum, White rot fungi Rhizopus arrhizus, Rhizopus oryzae, Rhizopus genus, Rhizopus genus, Rhizopus chinensis, Rhizopus var., Rhizopus oryzae, Rhizopus genus, Green cockroach, Fusarium oxysporum, Artemis sclerophylla, Corning Mildew, Trichoderma viride, Verticillium dahliae, Verticillium dahlia
15. Czapek's Agar (Caz's Agar)
Sucrose (sucrose) 30g NaNO3 3g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.7H2O 0.01g K2HPO4 1g
Agar (agar) 13g Distilled water (distilled water) 1000ml
pH 7.2
Scope of application: Streptomyces erythromycin, Aspergillus oryzae, Aspergillus oryzae, Aspergillus awamori, Aspergillus awamori, variant Aspergillus niger, Aspergillus niger, Aspergillus cyanosis, Aspergillus oryzae, Aspergillus flavus, Aspergillus flavus, Aspergillus flavus , Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Aspergillus oryzae, Aspergillus parasiticus, Aspergillus sojae, Aspergillus terreus, Aspergillus oryzae, Aspergillus oryzae, Aspergillus oryzae, Aspergillus niger, Ada Penicillium, Penicillium chrysogenum, Yellow green Mildew, Penicillium citrinum, Penicillium citrinum, Penicillium arctos, Penicillium funiculosum, Penicillium chrysogenum, Penicillium island, Penicillium chrysogenum, Specific penicillium, Penicillium spp., Penicillium chrysogenum Penicillium, Penicillium sinensis, Penicillium citrinum,
16. Concentrated Sucrose Czapek's (Concentrated Sugar Agar)
Sucrose (sucrose) 200g NaNO3 3g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.4H2O 0.01g K2HPO4 1g
Agar (agar) 13g Distilled water (distilled water) 1000ml
Scope of application: Aspergillus
17. Synthetic Potato Medium
20% Potato extract 1L Glucose (Glucose) 20g
KH2PO4 3g MgSO4.7H2O 1.5g
Vitanib B1 (thiamine) Trace (micro) Agar (agar) 15g
pH 6
Scope of application: Aspergillus oryzae, Aspergillus sojae, Aspergillus oryzae, Aspergillus oryzae, Aureobasidium pullulans, Beauveria bassiana, Botrytis cinerea, Cephalosporium acremonium, Brazilian hairy, long thorn shell,
Ball shell, olive coat shell, recurved shell, amber shell, round leaven shell, polyclostridium, bundled thorn spore, cucurbitaceae, Curvularia lunata, genus , the genus of the genus, the genus Fusarium, Fusarium oxysporum, Fusarium oxysporum (Lubao No.1), the long-spore of the white poplar, the shell of the bottle, the genus Metarhizium, the mucor, the coiled branch Mucor, two types of Mucor, Mucor pilus, Coccidiosus, Mucor, Mucor, Mucor, Mucor, Mucor, Mucor, Black lacquer Bacteria, Phytophthora capsici, Paecilomyces variabilis, Paecilomyces militaris, Paecilomyces lilacin, Paecilomyces sinensis, White rot fungi, Rhizopus arrhizus, Rhizopus, Rhizopus Rhizopus, Rhizopus, Rhizopus oryzae, Rhizopus chinensis, Rhizopus var., Rhizopus oryzae, Rhizopus oryzae, Rhizopus arrhizus, Rhizopus oryzae, Rhizopus genus, Rhizopus sylvestris, Rhizopus Species, Trichoderma virens, Trichoderma koningii, Trichoderma xylinum, Trichoderma koningii, Trichoderma, Trichoderma virens, Trichoderma viride, Verticillium dahliae, Rhododendron ssp., Agaricus bisporus, Big mushroom Illuminated fake honey ring , fungus, wrinkle fungus, hair fungus, big bald horse, long root mushroom, enoki mushroom, spine bamboo stalk, short skirt bamboo stalk, short skirt bamboo stalk northeast variant (tentative), long skirt bamboo raft, white ghost pen, Non-skid bamboo stalk, broad-skinned large-hole bacterium, convex round Ganoderma lucidum (Baizhi), Zizhi, Ganoderma lucidum (Hongzhi, Ganoderma lucidum, Japanese Ganoderma lucidum, Korean Ganoderma lucidum), ash tree flower, Hericium erinaceus, shiitake mushroom, shredded mushroom, clean Lich mushroom, leopard mushroom, jade, real mushroom, Anlu small toadstool, ghost hair needle, black handle leather toadstool, morel morel, conical morel, high morel, black vein sheep belly Bacteria, morel mushroom, mushroom, light hat yellow umbrella, sharp yellow umbrella, golden top ear, 榆 yellow mill, golden top mill, white yellow Pleurotus, Pleurotus ostreatus, Shime mushroom, Pleurotus ostreatus, pseudocyst Pleurotus ostreatus (ababa mushroom), Pseudostellaria serrata, true line Pleurotus, scalloped oyster mushroom, flat oyster mushroom, Florida spore-free Pleurotus, Florida Pleurotus, Pleurotus ostreatus, Pleurotus ostreatus, Pleurotus ostreatus, Pleurotus ostreatus, Acanthopanas , Pleurotus ostreatus, willow fungus, tree nest, Pleurotus ostreatus, delicious Pleurotus, Pleurotus ostreatus, Pleurotus ostreatus, Pink Pleurotus, Pleurotus ostreatus, Funnel-shaped Pleurotus Dry ear, big honing, side ear, cockroach, black mushroom, smoked red mushroom, schizophyllum, chicken longitudinal, golden ear, yellow fungus, white fungus, white fungus, amethyst grinding, silver mushroom, silk cover small foot Mushroom, straw mushroom
18. Corn Meal Medium
Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into
100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at
121 ° C for 30minutes . (20% corn flour bottling, 15 lbs sterilization for 30 minutes.)
19. Filter Paper Medium
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.7g NaCl 0.5g
Distilled water 1000ml A strip of filter paper 6x1cm (cm)
20. Soybean Cake Meal Agar (Soybean Cake Powder Agar)
10% infusion of soybean cake meal (10% soy cake powder dip) 1000ml
Glucose (glucose) 10g NaCl 2.5g21. Sawdust, Wheat-bran Medium (sawdust, bran medium)
Sawdust of broad leaf tree 75g Wheat bran 25g
Preparation:Mix these two ingredients well . Add appropriate water and blend
(the amount of water added may be becided by kneading with fingers ). Fill the
Mixing into broad mouth of bottles . Stick a hole on the filling, put the
Cotton pulg in bottle mouth .
Sapphire or broadleaf wood chips 75 g bran 25 g
Method: Add enough water to mix well. When the amount of water is pinched, it is advisable to have water drops between the fingers. Bottle and compact. Place a small hole in the center, plug the cotton plug, and sterilize 20 pounds for 1 hour.
22. Pine Block or Pine Sowdust Medium (pine bar or pine sawdust medium)
1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow
The biocks fully absorbing suger solution .Fill the biocks into broad month of
Park the bottle with paper . Autoclave at 20 lbs for 1 hr .
(Immerse 10x2x1 cm pine strips in 1-2% sucrose water. Make the strips suck enough sugar, do not overtighten the bottles, wrap them in paper, and sterilize them at 20 lbs for 1 hour.)
2. Pine Sawdust Medium
Pine Sawdust 75% Rice bran (rice) 23-24%
Socrose (sucrose) 1-2% Water (appropriate) water (appropriate amount)
Autoclave at 20 lbs for 1 hr. (20 lbs for 1 hour)
23. Rice Straw. Dung Medium (straw, horse dung medium)
Dried horse dung (or cow dung) (dry horse dung or cow dung) 65%
Died straw 35% Urea (urea) 0.5%
Calcium superhosphate (superphosphate) 0.7% Gypsum powder (gypsum powder) 1%
Ammonium sulphate (ammonium sulfate) 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation.
(water amount for accumulation fermentation)
24. Straw Rice Bran Medium (straw, rice bran medium)
Dried straw 75-80% Rice bran (rice bran) 20-25%
[Note]:Select no mouldy straw and cut them about 3 cm in length. Immerse them
In fresh water for 12 hr. Allow the straw fully (the amount of water added may
Be decided by kneading with fingers. It is right when a drop of water appeared
Between fingers). (
[Note]: Select mildewed straw, cut into small sections of about 3 cm, soak in water for 12 hours, so that it can fully absorb water, then pour water, add rice bran and water, and the amount of water should be separated by hand when there is water. . )
25. Rice Medium (rice medium)
Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave
At 121°C for 30 minutes (or fill 2g of rice into a 15x150mm tube). Add 6 ml of
Water. Autoclave at 121°C for 30 minutes.
(take 10 grams of rice, into a 50 ml vertebra bottle, 20-30 ml of water in 15 liters for 30 minutes, or take 2 grams of rice, put into a 15x150 mm test tube, add 6 ml of water, 15 lbs. 30 minutes.) 26. Filter Paper Medium
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.5g Yeast Extract 0.1g
Distilled Water (Distilled Water) 1000ml
Strip of filter paper 1x7 cm (filter strip 1x7 cm)
[Note]:Siddolve the above ingredients. Distribute the liqid medium into tubes
(each tube contains 1ml of the broth). Autoclave at 121°C for 30 minutes. Then
Put one or two sterilized strips of filter paper into a tube aseptically.
(Distribute the above solution into a solution and dispense into a test tube, 1 ml per tube, and sterilize for 15 minutes at 15 lb. Then use the sterilized filter paper strip (1 or 2) into the test tube.)
27. Yeast Extract-Medium Extract Agar I (Yeast Extract, Malt Extract Agar)
Yeast extract 10g Mall extract 10g
Glucose (glucose) 4g Distilled water (distilled water) 1000ml
Agar (agar) 20g pH 7.0
Scope of application: Streptomyces erythromycin
28. Yeast Extract Peptone (Yeast Extract, Peptone Agar)
Yeast extract 1g Multi-peptone 2g
Beef extract 1g Glucose 10g
Agar (agar) 20g Distilled water (distilled water) 1L
pH 7.0
29. Glucose Yeast Extract Starch Agar (glucose, yeast, starch agar)
Glucose (glucose) 10g Yeast extract (yeast extract) 10g
Starch (starch) 10g NaCl 5g
CaCO3 3g Agar (agar) 20g
Distilled water 1L pH 7.0
30. Wheat Bran Medium (Bran Medium)
Wheat bran (Bran) 36g (NH4)2HPO4 10g
K2HPO4 0.2g MgSO4·7H2O 0.1g
Agar (agar) 20g Distilled water (distilled water) 1L
pH 7.031. Yeast Sporulation Medium (yeast production of ascospores)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate 0.5% Glucose 0.062%
NaCl 0.062% Peptone (peptone) 0.25%
Biotin 2μg% Agar (agar) 2%
Trace element solution 1ml/100ml
[Note]: Trace element solution: MgSO4.7H2O 0.4%, NaCl 0.4%,
CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
(2) Gorodkowa Medium
Glucose (glucose) 0.1% Peptone (peptone) 1%
NaCl 0.5% Agar (agar) 2%
(3) McClary Medium
Glucose (glucose) 0.1% KCl 0.18%
Yeast juice 0.25% Sodium actate (sodium acetate) 0.82%
Agar (agar) 1.5%
32. BPY Medium (BPY Medium)
Beef extract 5g Prptone 10g
NaCl 5g Yeast extract 5g
Glucose (glucose) 5g Agar (agar) 1-2%
Distilled (distilled water) 1000ml pH 7.0
Scope of application: Escherichia coli (Escherichia coli), Pseudomonas putida
33. LB Medium (LB medium)
Yeast extact 5g Peptone 10g
NaCl 10g Agar (agar) 1-2%
Distilled water 1000ml pH 7.0
Scope of application: Escherichia coli (E. coli)
34. Penssay Broth (Penssay Broth)
Beef extract 1.5g Yeast extract 1.5g
Tryptone 5g NaCl 3.5g
Glucose (glucose) 1g K2HPO4.3H2O 4.8g
Distilled water 1000ml pH 7.2
35. Blond Agar (blood agar medium)
Peptone 10g Glucose 2g
NaCl 5g NaH2PO4 2.5g
Sodium citrate 5g Brain infusion 800ml
Pig heart infusion 200ml Sleep Blood 10%
pH 7.2
[Note]:Preparation of pig brain and pig heart infusion:
(1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled
Water up to 1000ml;
(2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to
1000ml;
(3) Keep the two infusions at 50°C for 1 hr , then boil for 15 minutes;
(4) Cool and filtrate through gauze .
[Note] Pig heart and pig brain dip juice method: (1) remove the heart and fat from the fresh pig heart, crush 100 grams of distilled water to 1000 ml; (2) remove the blood vessels from fresh pig brain, take 200 grams of distilled water to 1000 ml (3) Incubate at 50 ° C for 1 hour, then boil for 15 minutes; (4) After cooling, filter with gauze for later use. 36. Cellulose-decomposing Bacteria Synthetic Medium
NaCl 6g MgSO4.7H2O 0.1g
KH2PO4 0.5g CaCl2 0.1g
K2HPO4 2.0g Yeast extract 1.0g
(NH4)2SO4 2.0g Distilled water (distilled water) 1000ml
Filter paper strip: one strip (filter strip: one piece) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl
Cellulose 2% Agar 2%. (Slope medium filter strip with 2% agar and 2% sodium carboxymethylcellulose CMCNa)
37. Clostridium Pasreurianum Synthetic Medium (C. brevisii synthetic medium)
Glucose (glucose) 1% MnSO4 0.001%
KH2PO4 0.05% FeSO4 0.001%
K2HPO4 0.05% Yeast extract 0.1%
MgSO4.7H2O 0.02% Peptone (peptone) 0.01%
NaCl 0.001% CaCO3 0.5%
Distille water 100% pH 7.038. Yeast Extract-Medium Extract Agar II (Yeast Extract, Malt Extract Agar II)
Yeast extract 4g Mall extract 10g
Glucose (glucose) 4g Agar (agar) 17g
Distilled water (distilled) 1000ml pH 7.3
39. Oatemeal Agar (Oatmeal Agar)
Oatemeal (infusion) [oat flour infusion] 20g Agar (agar) 18%
Trace element solution 1ml
Distilled water up to (1000 ml with distilled water) 1000 ml pH 7.2
[Note]: Trace element solution:
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g Distilled water (distilled water) 100ml
40. Calcium Malate Agar (calcium malate agar)
Calium malate 10g K2HPO4 0.5g
NH4Cl 0.5g Glycerol (glycerol) 10g
Agar (agar) 15g Distilled water (distilled water) 1000ml
41. Thiobacillus Ferrooxidans Medium
(NH4)2SO4 0.15g KH2PO40.05g
KCl 0.05g MgSO4.7H2O 0.5g
Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g
Distilled water 1000ml pH 2.0
42. Thiobacillus Thiooxidans Medium
(NH4)2SO4 0.3g KH2PO4 3-4g
CaCl2 (anhydrous) [anhydrous] 0.25g MgSO4.7H2O 0.5g
FeSO4.7H2O 0.001g Distilled water (distilled water) 1000ml
pH 3.5-4
43. Plasma Substitute Medium
Sucrose (sucrose) 12.5-15% Peptone (peptone) 0.5%
KH2PO4 0.03% Na2HPO4.12H2O 0.14%
pH 7.0-7.2
44. Skim Milk Medium ATCC 183
Skim milk 100g deionized water 1000ml
Scope of application: Lactobacillus bulgaricus, Streptococcus thermophilus
45. Pea Agar (pea agar)
Glucose (glucose) 1% Peptone (peptone) 0.5%
NaCl 0.5% Agar (agar) 2.0%
Pea infusion (counted by NH2) [pea immersion juice (in NH2)] 12-14 mg∕100
pH (before sterilization) [Before elimination] 7.2-7.5
[Note]: Preparetion of pea infusion (preparation of pea sauce):
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water.
Adjust pH to 4.3-4.5 by using sulphuric acid. Add 30ml of chloroform for
Antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge
In order to avoid that the stopper fall off due to gas pressure.
Bottle at 37°C for 7-10 days. Filter the infusion, then boil the filtrate to
Escape the chloroform. Filter again Distribution and autoclave at 120°C for 30
Minutes. Store at cool contition .
(Weigh 900 grams of white peas, put in a 5 liter jar, add 3 liters of tap water, adjust the pH to 4.3-4.5 with sulfuric acid, add 30 ml of chloroform (preservative), add rubber stopper and tie with gauze (to prevent rushing) Remove), put in a 37 ° C constant temperature chamber for 7-10 days. Take out and filter with filter paper, boil the filtrate for 20 minutes to remove chloroform, and filter again with filter paper to remove the precipitate. The filtrate is divided and sterilized at 120 ° C for 30 minutes. Spare.) The standard quality of pea infusion as following:
Aminonitrogen (amino acid): 160-200 mg ∕ 100ml
Soluble phosphoruse: 400-600 μg∕ml
Color: auratus, transparent (golden, transparent)
Main markets
Australia, New Zealand, UK., Northern Europe, etc.
Features
ŸWeather resistant and durable
ŸLight and easy to handle, while being strong and robust at the same time.
ŸThe fence feet are removable which makes transportation and storage much easier and more efficient.
Temporary Fence Panel,Crowd Control Barrier,Traffic Barrier
DINGZHOU TIAN YILONG METAL PRODUCTS CO., LTD. , https://www.wiremeshsolution.com